Research Associate - Functional Genomics (Molekularbiologe/-biologin)

“Research for a life without cancer" is our mission at the German Cancer Research Center. We investigate how cancer develops, identify cancer risk factors and look for new cancer prevention strategies. We develop new methods with which tumors can be diagnosed more precisely and cancer patients can be treated more successfully. Every contribution counts – whether in research, administration or infrastructure. This is what makes our daily work so meaningful and exciting.

The Precision Sarcoma Research group at the German Cancer Research Center and the National Center for Tumor Diseases Heidelberg (Head: Dr. Priya Chudasama) is seeking for the next possible date a Research Associate - Functional Genomics.

We are looking for a qualified and motivated research associate to expand our international, inclusive and ambitious team to contribute to projects focused on better understanding of rare cancers called bone and soft-tissue sarcoma, a large and heterogeneous group of aggressive tumors affecting all age groups with urgent unmet medical need. Our group uses computational and functional genomics approaches to characterize tumor specimens and cell models to identify mechanisms of sarcomagenesis and new biology-guided treatments. More details on the team and specific on-going projects could be found here: https://www.dkfz.de/en/precision-sarcoma-research (http://www.dkfz.de/en/precision-sarcoma-research)

Your Tasks

The successful candidate will flexibly support different on-going projects in the lab in close collaboration with team members and external collaborators, and take on lab management and coordination duties. The methods and general responsibilities include, but are not limited to:

- Analysis of next-generation sequencing data derived from human sarcoma samples to identify genome, transcriptome and methylome alterations, if bioinformatics expertise is available
- CRISPR/Cas9 screens
- Molecular biology methods (sample preparation, cloning, sequencing, mutagenesis, PCR)
- Routine cell culture methods (culture of cell lines, production of lentiviruses, transduction of cell lines, different assays for hallmarks of cancer)
- Flow cytometry and cell sorting
- Protein detection (western blotting, immunoprecipitation, immunofluorescence)
- Comprehensive documentation and presentation of results in lab meetings and conferences
- Independent planning and coordination of project, analysis and interpretation of results, establishment of new methods, supervision of new lab members
- Manage key communication with collaborators, third-party service providers, and other internal departments
- Support laboratory management (ordering of reagents and consumables, maintaining inventories and lab databases, shipments)

Your Profile

- Master of Science, PhD or equivalent degree
- Extensive experience with cell culture and molecular biology methods
- Competence and experience in programming languages (R, Python), experience with analysis of next-generation sequencing data will be a plus
- Exceptional organizational and time management skills, willingness to support different projects in parallel, as well as to move between sites for use of different technology platforms
- True team player, high level of intrinsic motivation, reliability
- Excellent command of written and spoken English and effective oral and written presentation skills, German language skills would be helpful but not a must

Interested candidates should send applications with full CV with publications, references and cover letter explicitly mentioning the date of availability. Incomplete applications will not be considered. In case of urgent questions, please write an email to [email protected]. Please apply exclusively via the application portal.

We Offer

- Excellent framework conditions: state-of-the-art equipment and opportunities for international networking at the highest level
- 30 days of vacation per year
- Flexible working hours
- Remuneration according to TV-L incl. occupational pension plan and capital-forming payments
- Possibility of part-time work
- Family-friendly working environment
- Sustainable travel to work: subsidized Germany job ticket
- Unleash your full potential: targeted offers for your personal development to further develop your talents
- Our Corporate Health Management Program offers a holistic approach to your well-being

Are you interested?

Then become part of the DKFZ and join us in contributing to a life without cancer!

Contact:

Dr. Priya Chudasama
Telefon: +49 6221 42 1600 (https://jobs.dkfz.de/en/jobs/167745/+49%206221%2042%201600)

​Duration: The position is initially limited to 1 year with the possibility of prolongation.

Application Deadline: 04.06.2025

Applications by e-mail cannot be accepted.

We are convinced that an innovative research and working environment thrives on the diversity of its employees. Therefore, we welcome applications from talented people, regardless of gender, cultural background, nationality, ethnicity, sexual identity, physical ability, religion and age. People with severe disabilities are given preference if they have the same aptitude.

Notice: We are subject to the regulations of the Infection Protection Act (IfSG). Therefore, all our employees must provide proof of immunity against measles.

„Forschen für ein Leben ohne Krebs“ – das ist unsere Aufgabe am Deutschen Krebsforschungszentrum. Wir erforschen, wie Krebs entsteht, erfassen Krebsrisikofaktoren und suchen nach neuen Strategien, die verhindern, dass Menschen an Krebs erkranken. Wir entwickeln neue Methoden, mit denen Tumore präziser diagnostiziert und Krebspatient:innen erfolgreicher behandelt werden können. Jeder Beitrag zählt – ob in der Forschung, in der Administration oder der Infrastruktur. Das macht unsere tägliche Arbeit so bedeutungsvoll und spannend.

Die Abteilung Mikrobiologische Diagnostik sucht ab dem 01.04.2025 eine:n Biotechnologe:in / Molekularbiologe:in (w/m/d).

Wir suchen für die Mitarbeit in der Mikrobiologischen Diagnostik eine:n engagierte:n Biotechnologen:in oder Molekularbiologen:in mit abgeschlossenem Bachelor-Studium (BA) (w/m/d) mit einem starken Interesse an der Erreger-Diagnostik und Freude am Arbeiten im Team. Als eine Abteilung des Zentrums für Präklinische Forschung am Deutschen Krebsforschungszentrum (DKFZ) ist die Mikrobiologische Diagnostik insbesondere für die Durchführung der Gesundheitsüberwachung der Versuchstiere am DKFZ aber auch für die Untersuchung biologischer Materialien und Umweltproben zuständig.

Ihre Aufgaben:

- Mitarbeit in der Routine-Diagnostik der Mikrobiologischen Diagnostik
- Eigenständige Entwicklung und Validierung von (Multiplex-)qPCR-Tests für die mikrobiologische Diagnostik
- Neuentwicklung eines qPCR-Arrays (Array Cards) für die Hochdurchsatz-Analyse von Umweltproben (Environmental Health Monitoring)
- Anwendung von bioinformatischen Programmen zur Analyse von Sequenzdaten, Sonden- und Primerdesign
- Durchführung von molekularbiologischen Untersuchungen im Rahmen der Routine-Diagnostik (konventionelle PCR, qPCR, Multiplex-PCR)
- Erstellung von Validierungsberichten und Standardarbeitsanweisungen (SOPs)
- Optimierung des 'Environmental Health Monitoring' auf Ebene der Tierhaltung und auf Laborebene (Bearbeitung, Aufreinigung, qPCR-Analyse) zur Verbesserung der Aussagekraft, Sensitivität und Spezifität der Untersuchungen; laufende Anpassung der Erregerpalette
- Tierexperimentelle Arbeiten und Planung von Tierversuchen

Ihr Profil:

- Abgeschlossenes Bachelorstudium Fachrichtung Biotechnologie, Molekulare Biologie oder vergleichbarer Fachrichtung
- Erfahrungen mit molekularbiologischen Methoden insbesondere der Durchführung und Auswertung von Multiplex-qPCRs, sowie der Methodenetablierung und -validierung werden vorausgesetzt
- Erfahrungen mit anderen diagnostischen Methoden (bakteriologische, virologische und serologische Methoden) sind von Vorteil, aber keine Voraussetzung
- Erfahrungen mit tierexperimentellen Arbeiten und ein abgeschlossener FELASA-Kurs sind erwünscht
- Eigenverantwortliche, sorgfältige und strukturierte Arbeitsweise
- Hohe Eigeninitiative und Motivation, sich in neue Methoden einzuarbeiten
- Freude am Arbeiten im Team
- Sehr gute Deutsch- und Englischkenntnisse

Wir bitten um ein aussagekräftiges Anschreiben.

Unser Angebot:

- Hervorragende Rahmenbedingungen: modernste state-of-the-art Infrastruktur und Möglichkeit zum internationalen Austausch auf Spitzenniveau
- 30 Tage Urlaub
- Flexible Arbeitszeiten
- Vergütung nach TV-L inkl. betrieblicher Altersvorsorge und vermögenswirksamer Leistungen
- Möglichkeit zur Teilzeitarbeit
- Familienfreundliches Arbeitsumfeld
- Nachhaltig zur Arbeit: Vergünstigtes Deutschland-Jobticket
- Entfalten Sie Ihr volles Potenzial: gezielte Angebote für Ihre persönliche Entwicklung fördern Ihre Talente
- Unser betriebliches Gesundheitsmanagement bietet ein ganzheitliches Angebot für Ihr Wohlbefinden

Sie sind interessiert?

Dann werden auch Sie Teil des DKFZ und tragen gemeinsam mit uns zu einem Leben ohne Krebs bei!

Ihre Ansprechperson:
Dr. Katja Schmidt
Telefon: +49 6221/42-4299

​Befristung: Die Stelle ist zunächst auf 2 Jahre befristet. Eine Verlängerung ist möglich.

Bewerbungsschluss: 07.03.2025

Bewerbungen per E-Mail können leider nicht angenommen werden. ​
Bitte beachten Sie auch, dass wir per Post eingereichte Bewerbungen nicht zurückschicken können.

Wir sind davon überzeugt: ein innovatives Forschungs- und Arbeitsumfeld lebt von der Vielfalt seiner Beschäftigten. Daher freuen wir uns über Bewerbungen von talentierten Menschen, unabhängig von Geschlecht, kulturellem Hintergrund, Nationalität, ethnischer Zugehörigkeit, sexueller Identität, körperlichen Fähigkeiten, Religion und Alter. Menschen mit Schwerbehinderung werden bei gleicher Eignung bevorzugt.

Hinweis: Wir unterliegen den Vorschriften des Infektionsschutzgesetzes (IfSG). Deshalb müssen alle unsere Beschäftigten einen Immunitätsnachweis gegen Masern vorlegen.

“Research for a life without cancer" is our mission at the German Cancer Research Center. We investigate how cancer develops, identify cancer risk factors and look for new cancer prevention strategies. We develop new methods with which tumors can be diagnosed more precisely and cancer patients can be treated more successfully. Every contribution counts – whether in research, administration or infrastructure. This is what makes our daily work so meaningful and exciting.

Together with university partners at seven renowned partner sites, we have established the German Cancer Consortium (DKTK).

We are seeking for the Junior Research Group "Cancer Bioinformatics and Multiomics" at the DKTK partner site Essen / Düsseldorf a Postdoc - Single-Cell Sequencing and Bioinformatics.

Cancer Bioinformatics and Multiomics is a junior group located at the Universitätsklinikum Düsseldorf. We apply third-generation and single-cell sequencing technologies to address questions relating to aberrant transcriptional processes in diverse cancers.

Your Tasks

We are looking for a highly motivated postdoctoral scientist with a hybrid background in computational biology and single-cell/genomic science at the bench. The successful applicant will join a DFG-supported collaborative project to conduct single-cell in vivo CRISPR screens on gastric cancer patient-derived organoids transplanted into mouse models to search for epigenetic modulators of tumorigenesis and metastasis. Responsibilities include carrying out 10X Chromium single-cell sequencing and preparing CROP-seq libraries, identifying enriched sgRNAs via UMI sequencing, and performing downstream bioinformatic analysis such as gene-set enrichment analysis of hits, identification of somatic copy number alterations, elucidation of tissue-specific drivers of metastasis, determining the relationship between primary progenitor cells and their metastases, and assessing the role of the tumor microenvironment.

Your Profile

We are looking for exceptional candidates with a completed M.Sc. and Ph.D degree in the areas of molecular or cell biology, medical science, biochemistry, biophysics, computational biology, or similar programs. Preference will be given to those candidates with demonstrated experience performing and/or analyzing CRISPR screens or single-cell RNA sequencing data. The candidate should have previous experience in scRNA-seq data analysis tools such as Seurat, Scanpy, MAST, scCN, and MOFA for multiomic analyses. Since our team is international, strong written and spoken English is essential. German is desirable but not required. We value analytical thinking, flexibility regarding the tasks, responsibility, attention to detail, and above all teamwork skills.

We Offer

- Excellent framework conditions: state-of-the-art equipment and opportunities for international networking at the highest level
- Remuneration according to TV-L incl. occupational pension plan and capital-forming payments
- 30 days of vacation per year
- Flexible working hours
- Possibility of part-time work
- Family-friendly working environment
- Sustainable travel to work: subsidized Germany job ticket
- Our Corporate Health Management Program offers a holistic approach to your well-being
- Develop your full potential: access to the DKFZ International Postdoc Program and DKFZ Career Service with targeted offers for your personal development to further develop your talents

Are you interested?

Then become part of the DKFZ and join us in contributing to a life without cancer!

Contact:

Dr. David Koppstein
Phone: +49(0)211 811-7644

​Duration: The position is limited to 2 years.

Application Deadline: 28.02.2025
Applications by e-mail cannot be accepted.

Please also note that we cannot return applications submitted by post.

We are convinced that an innovative research and working environment thrives on the diversity of its employees. Therefore, we welcome applications from talented people, regardless of gender, cultural background, nationality, ethnicity, sexual identity, physical ability, religion and age. People with severe disabilities are given preference if they have the same aptitude.

Notice: We are subject to the regulations of the Infection Protection Act (IfSG). Therefore, all our employees must provide proof of immunity against measles.

“Research for a life without cancer" is our mission at the German Cancer Research Center. We investigate how cancer develops, identify cancer risk factors and look for new cancer prevention strategies. We develop new methods with which tumors can be diagnosed more precisely and cancer patients can be treated more successfully. Every contribution counts – whether in research, administration or infrastructure. This is what makes our daily work so meaningful and exciting.

The German Cancer Research Center is seeking for the next possible date a Postdoc in Cancer Epigenomics.

The Division of Cancer Epigenomics (https://www.dkfz.de/en/cancer-epigenomics), headed by Prof. Dr. Christoph Plass, is a dynamic research team focused on understanding epigenetic contributions to carcinogenesis and therapy resistance in solid tumors (lung [1], breast [2], prostate [3]) and leukemia [4] with a focus on state-of-the-art wet lab technologies and computational solutions [5,6]).

1. Llamazares-Prada M, Schwartz U, Pease DF, Pohl ST, Ackesson D, Li R, Behrendt A, Tamas R, Richter M, Muley T, et al: Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration. bioRxiv 2023.
2. Zhang G, Jurinovic V, Bartels S, Christgen M, Christgen H, Kandt LD, Raap M, Klein J, Katzke A-L, Hofmann W, et al: Development and validation of multivariate predictors of primary endocrine resistance to tamoxifen and aromatase inhibitors in luminal breast cancer reveal drug-specific differences. medRxiv 2023.
3. Gerhauser C, Favero F, Risch T, Simon R, Feuerbach L, Assenov Y, Heckmann D, Sidiropoulos N, Waszak SM, Hübschmann D, et al: Molecular Evolution of Early-Onset Prostate Cancer Identifies Molecular Risk Markers and Clinical Trajectories. Cancer Cell 2018, 34:996-1011 e1018.
4. Weichenhan D, Riedel A, Sollier E, Toprak UH, Hey J, Breuer K, Wierzbinska JA, Touzart A, Lutsik P, Bahr M, et al: Altered enhancer-promoter interaction leads to MNX1 expression in pediatric acute myeloid leukemia with t(7;12)(q36;p13). Blood Adv 2024, 8:5100-5111.
5. Scherer M, Singh I, Braun M, Szu-Tu C, Kardorff M, Rühle J, Frömel R, Beneyto-Calabuig S, Raffel S, Rodriguez-Fraticelli A, Velten L: Somatic epimutations enable single - cell lineage tracing in native hematopoiesis across the murine and human lifespan. bioRxiv 2024
6. Kelly K, Scherer M, Braun MM, Lutsik P, Plass C: EpiCHAOS: a metric to quantify epigenomic heterogeneity in single-cell data. Genome Biol 2024, 25:305.

Your Tasks

While the genetic components of therapy resistance have been extensively studied in various cancer types, non-genetic, including epigenetic, mechanisms of therapy resistance remain largely unexplored. Multiple mechanisms, including pre-existing persister cells or acquired resistance mediated by cell plasticity, will be tested in experimental model systems and patient-derived material using state-of-the-art single-cell epigenomic technologies.  

During this project, the candidate will use existing model systems (iPSCs, cancer cell lines, PDX, genetically engineered mouse models and patient-derived tumor material) to understand epigenetic dysregulation leading to non-genetic therapy resistance. Using CRISPR technology the candidate will investigate common mechanisms of cancer therapy resistance across the different cancer entities studied in the department, including solid tumors (lung, breast, prostate) or leukemia.

Specifically, the candidate will generate bulk (e.g., ACT-seq, ATAC-seq, WGS data) and single-cell epigenomic (scRNA-seq, scATAC-seq, scTAM-seq) data of the model systems with and without therapeutic pressure to understand epigenetic mechanisms of therapy resistance.

Your Profile

We are looking for a highly motivated molecular cancer biologist with a PhD degree who has an interest in cancer research and is able to work in teams with computational scientists.

The candidate should have the following skills:

- Good knowledge in molecular biology with a focus on (epi)genomic profiling
- Experience in CRISPR technologies
- Interest in interdisciplinary research
- Knowledge of epigenomic data analysis is a plus
- Fluency in English, German language skills are not required

We Offer

- Excellent framework conditions: state-of-the-art equipment and opportunities for international networking at the highest level
- Remuneration according to TV-L incl. occupational pension plan and capital-forming payments
- 30 days of vacation per year
- Flexible working hours
- Possibility of part-time work
- Family-friendly working environment
- Sustainable travel to work: subsidized Germany job ticket
- Our Corporate Health Management Program offers a holistic approach to your well-being
- Develop your full potential: access to the DKFZ International Postdoc Program and DKFZ Career Service with targeted offers for your personal development to further develop your talents

Are you interested?

Then become part of the DKFZ and join us in contributing to a life without cancer!

Contact:

Michael Scherer
Phone: +49 6221 42 3309

​Duration: The position is initially limited to 2 years with the possibility of prolongation.

Application Deadline: 26.02.2025

Applications by e-mail cannot be accepted.

Please also note that we cannot return applications submitted by post.

We are convinced that an innovative research and working environment thrives on the diversity of its employees. Therefore, we welcome applications from talented people, regardless of gender, cultural background, nationality, ethnicity, sexual identity, physical ability, religion and age. People with severe disabilities are given preference if they have the same aptitude.

Notice: We are subject to the regulations of the Infection Protection Act (IfSG). Therefore, all our employees must provide proof of immunity against measles.